Our Lab research revolves around HIV and drugs of abuse focusing mainly transcriptional and epigenetic aspects.

Dr. Tyagi received his PhD in the field of Molecular Biology of HIV from International center for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.

His main research areas of interest are:

1. Define the underlying molecular mechanisms that regulate HIV transcription and latency, and how can those mechanisms be used for therapeutic benefits.

2. Characterize the molecular mechanisms that different drugs of abuse modulate to enhance HIV replication and neurocognitive complications.

The main techniques we utilize are RNA-Seq, ChIP-Seq, ATAC-Seq Western blotting, cloning, flowcytometry, mutagenesis, human cell culture (primary and cell lines), protein expression and purification, enzymatic assays, light and fluorescent microscopy, confocal microscopy, transcriptomic and proteomic analyses, transcription and signal transduction assays, besides other usual molecular biology lab assays.